Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 36, Pages 31153-31160Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b09529
Keywords
antifouling; aptasensor; polyethylene glycol; polydopamine; cancer cell lysates; adenosine triphosphate
Funding
- Natural Science Foundation of Shandong Province of China [JQ201406]
- National Natural Science Foundation of China [21422504, 21675093]
- Taishan Scholar Program of Shandong Province of China [ts20110829]
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Detection of disease biomarkers within complex biological media is a substantial outstanding challenge because of severe biofouling and nonspecific adsorptions. Herein, a reliable strategy for sensitive and low-fouling detection of a biomarker, adenosine triphosphate (ATP) in biological samples was developed through the formation of a mixed self assembled sensing interface, which was constructed by simultaneously self-assembling polyethylene glycol (PEG) and ATP aptamer onto the self-polymerized polydopamine-modified electrode surface. The developed aptasensor exhibited high selectivity and sensitivity toward the detection of ATP, and the linear range was 0.1-1000 pM, with a detection limit down to 0.1 pM. Moreover, owing to the presence of PEG within the sensing interface, the aptasensor was capable of sensing ATP in complex biological media such as human plasma with significantly reduced nonspecific adsorption effect. Assaying ATP in real biological samples including breast cancer cell lysates further proved the feasibility of this biosensor for practical application.
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