Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 36, Pages 30480-30487Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b09717
Keywords
microfluidic paper-based devices; point-of-care (POC); distance-based detection; aptamer
Funding
- National Science Foundation of China [21325522, 21422506, 21435004, 21521004]
- National Basic Research Program of China [2013CB933703]
- Program for Changjiang Scholars and Innovative Research Teams in University [IRT13036]
- National Found for Fostering Talents of Basic Science (NFFTBS) [J1310024]
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An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification, and visual signal output based on aptamer/invertase-functionalized sepharose beads, cascaded enzymatic reactions, and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, whereas sepharose beads with larger size are excluded and stay in the upper zone. Finally, the released conjugate initiates cascaded enzymatic reactions and translates the target signal into a brown bar chart reading. By simply closing the device, the ID-oPAD enables a sample-in-answer-out assay within 30 min with visual and quantitative readout. Importantly, bound/free probe separation is achieved by taking advantage of the size difference between sepharose beads and cellulose pores, and the downstream enzymatic amplification is realized based on the compatibility of multiple enzymes with corresponding substrates. Overall, with the advantages of low-cost, disposability, simple operation, and visual quantitative readout, the ID-oPAD offers an ideal platform for point-of-care testing, especially in resource-limited areas.
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