Journal
AVIAN PATHOLOGY
Volume 46, Issue 5, Pages 541-547Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03079457.2017.1324198
Keywords
Salmonella enterica serovar Pullorum; biofilm; csgA; bcsA; mutant; pathogenicity
Categories
Funding
- National Natural Science Foundation of China [31572530]
- Special Fund for Agro-Scientific Research in the Public Interest [201303044]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Salmonella biofilm formation is important to environmental stress resistance and virulence. However, the roles of the csgA and bcsA genes, which affect curli protein and cellulose production, respectively, in Salmonella enterica serovar Pullorum, are unknown. Here we constructed deletions in the csgA and bcsA genes in S. enterica serovar Pullorum strain S6702 and evaluated several aspects of biofilm formation and virulence. Delta csgA showed decreased production of curli fimbriae, while Delta bcsA had reduced cellulose production. Both mutants had a reduced ability to form biofilms. Delta csgA was reduced in adhesion and invasion to HeLa cells and exhibited decreased intracellular proliferation in HD11 macrophages. Delta bcsA exhibited increased proliferation in HD11 cells and replicated better in chicken spleens, as compared to the wild-type strain. Delta csgA virulence was attenuated in assays involving oral challenge of one-day-old chickens.
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