Radioimmunochemical Studies for Development of a Solid-Phase Radioimmunoassay System Using Coated Beads for the Assessment of Human Chorionic Gonadotropin

A method was developed for immobilization of prepared human chorionic gonadotropin (beta-hCG) antisera on fresh plastic beads through a covalent immune bridge. Such covalent immune-coated antisera on plastic beads were used in the development of a noncentrifugation radioimmunoassay. I-125-beta-hCG was prepared using different oxidizing agents. The solid-phase RIA system developed was found to well compete with the conventional method.