Journal
ELECTROANALYSIS
Volume 29, Issue 9, Pages 2114-2124Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201700207
Keywords
L-lysine; Amperometry; Biosensor; Graphene; Poly(vinylferrocene)
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Funding
- Ankara University [14L0430005]
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A novel and sensitive amperometric biosensor for L-lysine determination based on a glassy carbon electrode (GCE) modified with graphene (GR) and redox polymer poly(vinylferrocene) (PVF) was constructed. L-lysine--oxidase was immobilized onto the modified GCE by a glutaraldehyde/bovine serum albumin cross-linking procedure. SEM, CV and EIS were used for the characterization of the surface morphology and stepwise fabrication processes of PVF/GR composite. Optimal composition of the biosensor and experimental conditions that affect the performance of the biosensor are discussed. The effect of buffer pH on biosensor response was studied in detail over a wide pH range. L-lysine biosensor displayed a linear range of 9.9x10(-7) - 3.1x10(-4) M with a low detection limit of 2.3x10(-7) M and K-M(app) value of 0.4mM. The L-lysine biosensor was tested using pharmaceutical sample and cheese with satisfactory results.
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