Journal
BIOCONTROL SCIENCE AND TECHNOLOGY
Volume 27, Issue 8, Pages 940-951Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2017.1366423
Keywords
Infectivity; granular formulation; plaster of Paris; white grub; pelletiser
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Funding
- SIP-IPN (Secretaria de Investigacion y Posgrado, Instituto Politecnico Nacional) project [20160475]
- CONACYT (Consejo Nacional de Ciencia y Tecnologia) [336753]
- [248261]
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The low survival time and diminished infectivity by entomopathogenic nematodes (EPNs) from granular formulations limit their efficiency against agricultural insect pests. This study determined the benefit of pre-acclimating infective juveniles (IJs) of Steinernema glaseri (NJ-43 strain) on extending their mean survival time (STm) in diatomaceous earth (DE) pellets and increasing their infectivity against Phyllophaga vetula. The IJs were reared in Galleria mellonella larvae placed in Petri dishes containing plaster of Paris (PP) or modified White traps (WTs). Pelletisation was performed in a machine operating on the principle of laminar flow using DE Celite (R) 209. Pellets were stored at room temperature (23 +/- 3 degrees C) and high relative humidity (96-100%). IJs harvested from WTs between the 3rd and the 5th days after the onset of emergence were more infective on P. vetula and pre-acclimation of S. glaseri in PP increased significantly its STm in the pellets; from 23.1 to 34.5 days, compared with nonpre- acclimatised IJs from WTs. However, juveniles with or without pre-acclimation formulated in DE pellets failed to achieve significant control of P. vetula. These results are discussed in light of the relationship between EPN survival and host infection by EPNs with possible effects of the formulation in DE pellets.
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