Journal
CARBOHYDRATE RESEARCH
Volume 449, Issue -, Pages 114-119Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2017.07.009
Keywords
Chondroitin sulfate; Glycosaminoglycan; Acid hydrolysis; Oligosaccharide; HPLC-MSn
Funding
- National Natural Science Foundation of China [31301431]
- Public Science and Technology Research Funds Projects of Ocean [201505029]
- National Key Technology Research and Development Program of China [2014BAD04B09]
- China Postdoctoral Science Foundation [2016M591418]
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Because the glycosidic linkage of uronic acid is most resistant to acid, oligosaccharides may be formed during the acid hydrolysis of acidic polysaccharides. To take chondroitin sulfate (CS) as an example of acidic polysaccharides, the present study characterized the oligosaccharides released through acid hydrolysis and demonstrated their usefulness for structural confirmation. Acid hydrolysates of commercial standard CSs from shark cartilage and porcine bone were elucidated using HPLC-MSn after 1-phenyl-3methyl-5-pyrazolone (PMP) derivatization, and altogether 11 di-, tri- and tetra-saccharides with or without sulfate/acetyl groups were identified by their multi-stage mass spectra. Meanwhile the trends of reaction yields of these oligosaccharides alone with trifluoroacetic acid (TFA) concentrations (0.1-2.0 M) were investigated, and 0.2 M TFA was recommended. Then three real samples, sturgeon backbone, porcine trachea and sea cucumber were analyzed, and their CSs were identified by detection of characteristic oligosaccharide fragments. The present study indicated that acid hydrolysis could provide information for acetyl substitution, sulfation and glycosidic linkages, and was helpful for the structural analysis of acidic polysaccharides. (C) 2017 Published by Elsevier Ltd.
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