Journal
ZEBRAFISH
Volume 14, Issue 1, Pages 73-76Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2016.1326
Keywords
zebrafish; mutant; in vitro translation
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Funding
- Simons Center for the Social Brain at MIT
- Pitt Hopkins Research Foundation
- NSF [NSF IOS-1258087]
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Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
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