Journal
ZEBRAFISH
Volume 14, Issue 3, Pages 216-222Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2016.1400
Keywords
electronic cigarettes; 1,2-propanediol (propylene glycol); zebrafish; early development; behavior
Categories
Funding
- RJR-Leon Golberg Postdoctoral Fellowship
Ask authors/readers for more resources
The use of electronic cigarettes (e-cigarettes) is increasing as an alternative to tobacco burning cigarettes; however, their safety remains to be fully determined. The long-term effects of e-cigarettes are unknown, including the effects of maternal e-cigarette use on pre-and postnatal development. Additional research on the safety of e-cigarettes is needed. Especially useful would be information from high-and moderate-throughput economic model systems. This study investigates the effects of 1,2-propanediol, which was identified as the main component of e-cigarette liquid, on early development of zebrafish (an in vivo high-throughput model system that was recently proposed for the study of tobacco cigarette and e-cigarette toxicity). Zebrafish embryos were exposed to 1.25% or 2.5% 1,2-propanediol from 6 to 72 h post-fertilization (hpf). We show that exposure to 1,2-propanediol did not significantly affect mortality. Hatching success was significantly lower in 2.5% 1,2-propanediol-exposed embryos at 48 hpf, but at 72 hpf no significant differences were noted. Moreover, exposure to 1,2-propanediol reduced growth and increased the incidence of string heart, pericardial edema, and yolk sac edema. Most importantly, developmental exposure to 1.25% 1,2-propanediol caused hyperactive swimming behavior in larvae. This study demonstrates that 1,2-propanediol has adverse impacts on early development in zebrafish.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available