Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 158, Issue -, Pages 203-212Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.06.046
Keywords
Poly-glycolic acid; Poly-lactic acid; Poly-4-hydroxybutyrate; Poly-caprolactone; Polymers; Tissue engineering
Funding
- Swiss National Science Foundation [310030_143992]
- Forschungskredit Candoc of the University of Zurich
- Forschungskredit Postdoc of the University of Zurich
- Foundation for Research in Science and the Humanities at the University of Zurich
- Alfred and Anneliese Sutter-Stottner-Foundation
- Swiss National Science Foundation (SNF) [310030_143992] Funding Source: Swiss National Science Foundation (SNF)
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Biodegradable scaffold matrixes form the basis of any in vitro tissue engineering approach by acting as a temporary matrix for cell proliferation and extracellular matrix deposition until the scaffold is replaced by neo-tissue. In this context several synthetic polymers have been investigated, however a concise systematic comparative analyses is missing. Therefore, the present study systematically compares three frequently used polymers for the in vitro engineering of extracellular matrix based on poly-glycolic acid (PGA) under static as well as dynamic conditions. Ultra-structural analysis was used to examine the polymers structure. For tissue engineering (TE) three human fibroblast cell lines were seeded on either PGA-poly-4-hydroxybutyrate (P4HB), PGA-poly-lactic acid (PLA) or PGA-poly-caprolactone (PCL) patches. These patches were analyzed after 21 days of culture qualitative by histology and quantitative by determining the amount of DNA, glycosaminoglycan and hydroxyproline. We found that PGA-P4HB and PGA-PLA scaffolds enhance tissue formation significantly higher than PGA-PCL scaffolds (p < 0.05). Polymer remnants were visualized by polarization microscopy. In addition, biomechanical properties of the tissue engineered patches were determined in comparison to native tissue. This study may allow future studies to specifically select certain polymer starter matrices aiming at specific tissue properties of the bioengineered constructs in vitro. (C) 2017 Elsevier B.V. All rights reserved.
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