Journal
BONE
Volume 103, Issue -, Pages 308-317Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2017.07.028
Keywords
Osteoarthritis; Sulforaphane; SPX-01; Cartilage; STR/Ort
Categories
Funding
- BBSRC [BB/I014608/1]
- Arthritis Research UK [20413, 20581, 20039, 19205]
- Biotechnology and Biological Sciences Research Council [BB/I014608/1] Funding Source: researchfish
- Versus Arthritis [20859, 20039, 20581, 19205, 20413] Funding Source: researchfish
- BBSRC [BB/I014608/1] Funding Source: UKRI
- EPSRC [EP/I02249X/1] Funding Source: UKRI
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Osteoarthritis (OA), affecting joints and bone, causes physical gait disability with huge socio-economic burden: treatment remains palliative. Roles for antioxidants in protecting against such chronic disorders have been examined previously. Sulforaphane is a naturally occurring antioxidant. Herein, we explore whether SFX-01 (R), a stable synthetic form of sulforaphane, modifies gait, bone architecture and slows/reverses articular cartilage destruction in a spontaneous OA model in STR/Ort mice. Sixteen mice (n = 8/group) were orally treated for 3 months with either 100 mg/kg SFX-01 (R) or vehicle. Gait was recorded, tibiae were microCT scanned and analysed. OA lesion severity was graded histologically. The effect of SFX-01 (R) on bone turnover markers in vivo was complemented by in vitro bone formation and resorption assays. Analysis revealed development of OA-related gait asymmetry in vehicle-treated STR/Ort mice, which did not emerge in SEX-01 (R)-treated mice. We found significant improvements in trabecular and cortical bone. Despite these marked improvements, we found that histologically-graded OA severity in articular cartilage was unmodified in treated mice. These changes are also reflected in anabolic and anti-catabolic actions of SEX-01 (R) treatment as reflected by alteration in serum markers as well as changes in primary osteoblast and osteoclast-like cells in vitro. We report that SFX-01 (R) improves bone microarchitecture in vivo, produces corresponding changes in bone cell behaviour in vitro and leads to greater symmetry in gait, without marked effects on cartilage lesion severity in STR/Ort osteoarthritic mice. Our findings support both osteotrophic roles and novel beneficial gait effects for SFX-01 (R) in this model of spontaneous OA. (C) 2017 The Authors. Published by Elsevier Inc.
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