Journal
ACS NANO
Volume 11, Issue 10, Pages 9814-9824Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.7b03131
Keywords
small-angle X-ray scattering; reentrant condensation; crystallization; resolubilization; like charge attraction; bridging interactions; sv40
Categories
Funding
- Kaye Einstein Fellowship Foundation
- Israel Science Foundation [656/17]
- US Israel Binational Science Foundation [2016311]
- FTA-Hybrid Nanomaterials Program of the Planning and Budgeting Committee of the Israel Council of Higher Education
- NIH [1R01AI118933]
Ask authors/readers for more resources
Crystallization is a fundamental and ubiquitous process that is well understood in the case of atoms or small molecules, but its outcome is still hard to predict in the case of nanoparticles or macromolecular complexes. Controlling the organization of virus nanoparticles into a variety of 3D supramolecular architectures is often done by multivalent ions and is of great interest for biomedical applications such as drug or gene delivery and biosensing, as well as for bionanomaterials and catalysis. In this paper, we show that slow dialysis, over several hours, of wild-type Simian Virus 40 (wt SV40) nanoparticle solution against salt solutions containing MgCl2, with or without added NaCl, results in wt SV40 nanoparticles arranged in a body cubic center crystal structure with Im3m space group, as a thermodynamic product, in coexistence with soluble wt SV40 nanoparticles. The nanoparticle crystals formed above a critical MgCl2 concentrations. Reentrant melting and resolubilization of the virus nanoparticles took place when the MgCl2 concentrations passed a second threshold. Using synchrotron solution X-ray scattering we determined the structures and the mass fraction of the soluble and crystal phases as a function of MgCl2 and NaCl concentrations. A thermodynamic model, which balances the chemical potentials of the Mg2+ ions in each of the possible states, explains our observations. The model reveals the mechanism of both the crystallization and the reentrant melting and resolubilization and shows that counterion entropy is the main driving force for both processes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available