Journal
CELL RESEARCH
Volume 27, Issue 10, Pages 1231-1242Publisher
SPRINGERNATURE
DOI: 10.1038/cr.2017.106
Keywords
epigenetics; 5-hydroxylmethylcytosine; cell-free DNA; cancer diagnostics
Categories
Funding
- National Natural Science Foundation of China [31571327, 91631111]
- National Institutes of Health [U01 CA154209]
- Department of Defense [W81XWH1110287]
- U.S. Department of Defense (DOD) [W81XWH1110287] Funding Source: U.S. Department of Defense (DOD)
Ask authors/readers for more resources
5-Hydroxymethylcytosine (5hmC) is an important mammalian DNA epigenetic modification that has been linked to gene regulation and cancer pathogenesis. Here we explored the diagnostic potential of 5hmC in circulating cell-free DNA (cfDNA) using a sensitive chemical labeling-based low-input shotgun sequencing approach. We sequenced cell-free 5hmC from 49 patients of seven different cancer types and found distinct features that could be used to predict cancer types and stages with high accuracy. Specifically, we discovered that lung cancer leads to a progressive global loss of 5hmC in cfDNA, whereas hepatocellular carcinoma and pancreatic cancer lead to disease-specific changes in the cell-free hydroxymethylome. Our proof-of-principle results suggest that cell-free 5hmC signatures may potentially be used not only to identify cancer types but also to track tumor stage in some cancers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available