Journal
ACS OMEGA
Volume 2, Issue 10, Pages 6975-6983Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.7b01029
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Funding
- FAPESP [2013/14262-7, 2014/16789-5]
- CNPq
- CAPES (Brazil)
- Embrapa-Rede Agronano
- MCTI SisNano
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [14/16789-5] Funding Source: FAPESP
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We report the fabrication of immunosensors based on nanostructured mats of electrospun nanofibers of polyamide 6 and poly(allylamine hydrochloride) coated either with multiwalled carbon nanotubes (MWCNTs) or gold nanoparticles (AuNPs), whose three-dimensional structure was suitable for the immobilization of anti-CA19-9 antibodies to detect the pancreatic cancer biomarker CA19-9. Using impedance spectroscopy, the sensing platform was able to detect CA19-9 with a detection limit of 1.84 and 1.57 U mL(-1) for the nanostructured architectures containing MWCNTs and AuNPs, respectively. The high sensitivity achieved can be attributed to the irreversible adsorption between antibodies and antigens, as confirmed with polarization-modulated infrared reflection absorption spectroscopy. The adsorption mechanism was typical Langmuir-Freundlich processes. The high sensitivity and selectivity of the immunosensors were also explored in tests with blood serum from patients with distinct concentrations of CA19-9, for which the impedance spectra data were processed with a multidimensional projection technique. The robustness of the immunosensors in dealing with patient samples without suffering interference from analytes present in biological fluids is promising for a simple, effective diagnosis of pancreatic cancer at early stages.
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