Simultaneous Determination of Na+, K+, Ca2+, Mg2+ and Cl- in Unstimulated and Stimulated Human Saliva Using All Solid State Multisensor Platform

Human saliva is one of the body fluids which collection method is relatively simple and non-invasive. The article is dedicated to assess concentration (activity) of Na+, K+, Ca2+, Mg2+ and Cl- in fresh, unstimulated or stimulated human saliva samples using single solid contact ion-selective electrodes with conventional reference electrode and self-made multisensor platform (MP) equipped with ion-selective membranes for Na+, K+, Ca2+, Mg2+ and Cl- and reference electrode made in solid state technology, based on dispersed KCl in the polymer. Both kind of electrodes, single ISE and miniaturized electrodes in multisensor platform (ISE-MP) were made of glassy carbon. The electrode surfaces have been modified by conductive polymer (PEDOT) layer deposition; with the exception of Cl- electrode, in which conducting polymer was not applied. Potentiometric measurements were used to compare the changes of the ionic composition in various samples of saliva.