Journal
BIOMATERIALS
Volume 143, Issue -, Pages 57-64Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2017.07.031
Keywords
3D culture; Gelatin methacrylate; Spheroid; Lens
Funding
- California Institute for Regenerative Medicine
- UC Berkeley Vision Science Program [NEI T32EY007043, NEI R01 EY013849]
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Cells form hollow, spheroidal structures during the development of many tissues, including the ocular lens, inner ear, and many glands. Therefore, techniques for in vitro formation of hollow spheroids are valued for studying developmental and disease processes. Current in vitro methods require cells to self organize into hollow morphologies; we explored an alternative strategy based on cell growth in predefined, spherical scaffolds. Our method uses sacrificial, gelatin microbeads to simultaneously template spherical chambers within a hydrogel and deliver cells into the chambers. We use mouse lens epithelial cells to demonstrate that cells can populate the internal surfaces of the chambers within a week to create numerous hollow spheroids. The platform supports manipulation of matrix mechanics, curvature, and biochemical composition to mimic in vivo microenvironments. It also provides a starting point for engineering organoids of tissues that develop from hollow spheroids. (C) 2017 Elsevier Ltd. All rights reserved.
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