Journal
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume 55, Issue 10, Pages 1517-1522Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/cclm-2016-0803
Keywords
aluminum; inductively coupled plasma mass spectrometry; potassium; reference method; serum
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Funding
- Special Project Plan for Scientific and Technological Basic Work
- National Natural Science Foundation of China [2013FY113800, 81301488]
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Background: Potassium is an important serum ion that is frequently assayed in clinical laboratories. Quality assurance requires reference methods; thus, the establishment of a candidate reference method for serum potassium measurements is important. Methods: An inductively coupled plasma mass spectrometry (ICP-MS) method was developed. Serum samples were gravimetrically spiked with an aluminum internal standard, digested with 69% ultrapure nitric acid, and diluted to the required concentration. The K-39/Al-27 ratios were measured by ICP-MS in hydrogen mode. The method was calibrated using 5% nitric acid matrix calibrators, and the calibration function was established using the bracketing method. Results: The correlation coefficients between the measured K-39/Al-27 ratios and the analyte concentration ratios were >0.9999. The coefficients of variation were 0.40%, 0.68%, and 0.22% for the three serum samples, and the analytical recovery was 99.8%. The accuracy of the measurement was also verified by measuring certified reference materials, SRM909b and SRM956b. Comparison with the ion selective electrode routine method and international inter-laboratory comparisons gave satisfied results. Conclusions: The new ICP-MS method is specific, precise, simple, and low-cost, and it may be used as a candidate reference method for standardizing serum potassium measurements.
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