Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 23, Issue 21, Pages 3850-3863Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v23.i21.3850
Keywords
sodium selenite; dextran sulfate sodium; chronic colitis
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Funding
- National Natural Science Foundation of China [31370921]
- Natural Science Foundation of Liaoning province [2015020515]
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AIM To assess the effect of sodium selenite on the severity of dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice. METHODS Mice were randomly divided into four groups (n = 10/group): normal group, selenium (Se) group, chronic colitis group, and Se + chronic colitis group. The mice were sacrificed on day 26. Survival rates, clinical symptoms, colon length, and histological changes were determined. The percentages and absolute numbers of immune system cells in the lamina propria lymphocytes (LPL) of the colon, the expression of mRNA in colon tissue, and the concentrations of Th1, Th17, and Treg cytokines in LPL from the large intestine, were measured. RESULTS Se significantly ameliorated the symptoms of colitis and histological injury (P < 0.05 each), increasing the proportions of neutrophils and CD4(+) CD25(+) T cells (P < 0.05 each) and decreasing the proportions of gamma delta T cells, CD4(+), CD4(+) CD44(+), and CD4(+) CD69(+) T cells in LPL (P < 0.05 each). Moreover, Se reduced the expression of IL-6, IFN-gamma, IL-17A, IL-21, T-bet, and ROR gamma t (P < 0.05 each), but enhanced the expression of IL-10 and Foxp3 (P < 0.05 each). CONCLUSION These results suggest that Se protects against DSS-induced chronic colitis perhaps by increasing the number of CD4(+) CD25(+) Tregs that suppress the secretion of proinflammatory cytokines and populations of Th1, Th17, and gamma delta T cells.
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