Journal
BIOCHEMISTRY
Volume 56, Issue 39, Pages 5165-5170Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.7b00529
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Funding
- Howard Hughes Medical Institute
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Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the photon budget require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, protein engineering, and organic synthesis that have allowed a triumphant return of chemical fluorophores to modern biological imaging.
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