Journal
BIO-PROTOCOL
Volume 7, Issue 19, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2575
Keywords
SEM; Scanning Electron Microscopy; Olive; Flowering; Inflorescence; Meristem; Bud
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Here we present a protocol that describes how to image the structure of the olive axillary bud meristem with a scanning electron microscope (SEM) in order to characterize its identity and developmental stage. Briefly, the specimen is fixed with glutaraldehyde, saturated with ethanol, dried in a critical point dryer (CPD) system, dissected, coated with a conducting material and imaged with a scanning electron microscopy (SEM).
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