Journal
CHEMBIOCHEM
Volume 18, Issue 19, Pages 1888-1892Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201700406
Keywords
coiled coils; protein cages; protein design; self-assembly; symmetry
Funding
- Army Research Office [W911NF-11-1-0251, W911NF-16-1-0147]
- Howard Hughes Foundation
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The organization of proteins into new hierarchical forms is an important challenge in synthetic biology. However, engineering new interactions between protein subunits is technically challenging and typically requires extensive redesign of protein-protein interfaces. We have developed a conceptually simple approach, based on symmetry principles, that uses short coiled-coil domains to assemble proteins into higher-order structures. Here, we demonstrate the assembly of a trimeric enzyme into a well-defined tetrahedral cage. This was achieved by genetically fusing a trimeric coiled-coil domain to its C terminus through a flexible polyglycine linker sequence. The linker length and coiled-coil strength were the only parameters that needed to be optimized to obtain a high yield of correctly assembled protein cages.
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