Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 27, Issue 38, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201702741
Keywords
biophotonics; diagnostics; immunoassays; nanocomposites; optical biosensors; paper-based devices
Categories
Funding
- CERCA programme/Generalitat de Catalunya
- H-EU (INTCATCH Project) [689341]
- Generalitat de Catalunya [2014 SGR 260]
- Severo Ochoa Program (MINECO) [SEV-2013-0295]
- Royal Golden Jubilee Project of the Thailand Research Fund
- King Mongkut's University of Technology Thonburi [PHD/0109/2553]
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Immunoassays are nowadays a crucial tool for diagnostics and drug development. However, they often involve time-consuming procedures and need at least two antibodies in charge of the capture and detection processes, respectively. This study reports a nanocomposite based on graphene oxidecoated nanopaper (GONAP) facilitating an advantageous immunosensing platform using a single antibody and without the need for washing steps. The hydrophilic, porous, and photoluminescence-quenching character of GONAP allows for the adsorption and quenching of photoluminescent quantum dots nanocrystals complexed with antibodies (Ab-QDs), enabling a ready-to-use immunosensing platform. The photoluminescence is recovered upon immunocomplex (antibody-antigen) formation which embraces a series of interactions (hydrogen bonding, electrostatic, hydrophobic, and Van der Waals interactions) that trigger desorption of the antigen-Ab-QD complex from GONAP surface. However, the antigen is then attached onto the GONAP surface by electrostatic interactions leading to a spacer (greater than approximate to 20 nm) between Ab-QDs and GONAP and thus hindering nonradiative energy transfer. It is demonstrated that this simple-yet highly sensitive-platform represents a virtually universal immunosensing approach by using small-sized and big-sized targets as model analytes, those are, human-IgG protein and Escherichia coli bacteria. In addition, the assay is proved effective in real matrices analysis, including human serum, poultry meat, and river water. GONAP opens the way to conceptually new paper-based devices for immunosensing, which are amenable to point of care applications and automated diagnostics.
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