A Novel Silver Coating for Antigen-Microarray Preparation Suitable for Application on Antibody Recognition

The present paper reports the development of a novel silver nanoparticles-derivatized polystyrene substrate useful for for protein microarray application. The silvered coating was prepared by a novel photochemical method. Various coatings were tested and characterized by means of chemical-physical methods. It was demonstrated that these methods are useful for the preparation of protein microarray for the analysis of human antibody by sandwich methods of fluorescence immunoassay (Alexa labeled). Comparative studies were carried out with different printing buffers and various surface derivatization to find the optimal conditions for antigen immobilization. Therein described assay, the human myeloperoxidase antigen (MPO) was quantitative detected directly from human serum. The cross-reactivity was here evaluated by reaction with recombinant human tissue transglutaminase antigen (htTG), a linearity response from 20 to 2000 ng/mu l was investigated by human IgG probes. The data indicate an increase of both signal-to-noise ratio value and reproducibility for the silver nanoparticle coating respect the value obtained for the polystyrene commercial slides.