Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 20, Pages 10734-10743Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b01730
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Funding
- National Institutes of Health (NIH) [R21GM122633, P41GM103490, S10OD018530]
- U.S. Department of Energy [DE-SC0015662]
- U.S. Department of Energy (DOE) [DE-SC0015662] Funding Source: U.S. Department of Energy (DOE)
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To overcome the challenges in the analysis of protein glycosylation, we have developed a comprehensive and universal tool through permethylation of glycopeptides and their tandem mass spectrometric analysis. This method has the potential to simplify glycoprotein analysis by integrating glycan sequencing and glycopeptide analysis in a single experiment. Moreover, glycans with unique glycosidic linkages, particularly from prokaryotes, which are resistant to enzymatic or chemical release, could also be detected and analyzed by this methodology. Here we present a strategy for the permethylation of intact glycopeptides, obtained via controlled protease digest, and their characterization by using advanced mass spectrometry. We used bovine RNase B, human transferrin, and bovine fetuin as models to demonstrate the feasibility of the method. Remarkably, the glycan patterns, glycosylation site, and their occupancy by N-glycans are all detected and identified in a single experimental procedure. Acquisition on a high resolution tandem-MS system with fragmentation methodologies such as high-energy collision dissociation (HCD) and collision induced dissociation (CID), provided the complete sequence of the glycan structures attached to the peptides. The behavior of 20 natural amino acids under the basic permethylation conditions was probed by permethylating a library of short synthetic peptides. Our studies indicate that the permethylation imparts simple, limited, and predictable chemical transformations on peptides and do not interfere with the interpretation of MS/MS data. In addition to this, permethylated O-glycans in unreduced form (released by beta elimination) were also detected, allowing us to profile O-linked glycan structures simultaneously.
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