Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 20, Pages 11151-11158Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b03708
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Funding
- National Natural Science Foundation of China [21425518, 21405022, 21675034]
- National Basic Research Priorities Program of China [2013CB911201]
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Developing cost-effective approaches based on hydrophilic interaction liquid chromatography (HILIC) has been the main tendency for low -abundance glycopeptides capture before LC-MS/MS analysis. Carbohydrates with outstanding bio compatibility and hydrophilicity are ubiquitous in the kingdoms of animal and plant and could be a wonderful choice as functional groups for glycopeptides enrichment. In this work, glucose-6-phosphate, as one of the indispensable cogs in pivotal metabolic wheels of life, was chosen as functionalized groups to be grafted onto the surface of Fe3O4 microspheres via one-step surface fabrication strategy. The acquired hydrophilic Fe3O4@G6P microspheres showed superior enrichment performance for glycopeptides with high sensitivity (0.5 fmol/mu L) and high selectivity (1:100) and good repeatability (10 times at least). Furthermore, the Fe3O4@G6P microspheres also exhibited enrichment ability for glycopeptides in different biosamples. A total of 243 glycopeptides assigned to 92 glycoproteins and 183 glycopeptides corresponding to 74 different glycoproteins was identified from merely 2 mu L of serum and saliva, respectively.
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