Journal
BRAIN RESEARCH BULLETIN
Volume 134, Issue -, Pages 273-282Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2017.08.010
Keywords
Rhesus monkey; Animal model; Kainic acid; Temporal lobe epilepsy; EEG; Amygdala
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Funding
- National Natural Science Foundation of China [61571196, 81501046]
- Science and Technology Program of Guangdong Province, China [2014A020212179, 208]
- National Science and Technology Support Program of China [2014BAI03B01]
- Guangdong Provincial Clinical Medical Center for Neurosurgery [2013B020400005]
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Objective: Temporal lobe epilepsy (TLE) is a common type of acquired epilepsy refractory to medical treatment. As such, establishing animal models of this disease is critical to developing new and effective treatment modalities. Because of their small head size, rodents are not suitable for comprehensive electroencephalography (EEG) evaluation via scalp or subdural electrodes. Therefore, a larger primate model that closely recapitulates signs of TLE is needed; here we describe a rhesus monkey model resembling chronic TLE. Methods: Eight monkeys were divided into two groups: kainic acid (KA) group (n = 6) and saline control group (n = 2). Intra-amygdala KA injections were performed biweekly via an Ommaya device until obvious epileptiform discharges were recorded. Video-EEG recording was conducted intermittently throughout the experiment using both scalp and subdural electrodes. Brains were then analyzed for Nissl and glial fibrillary acid protein (GFAP) immunostaining. Results: After 2-4 injections of KA (approximately 1.2-2.4 mg, 0.12-0.24 mg/kg), interictal epileptiform discharges (IEDs) were recorded in all KA-treated animals. Spontaneous recurrent seizures (SRSs) accompanied by symptoms mimicking temporal lobe absence (undetectable without EEG recording), but few mild motor signs, were recorded in 66.7% (four of six) KA-treated animals. Both IEDs and seizures indicated a primary epileptic zone in the right temporal region and contralateral discharges were later detected. Segmental pyramidal cell loss and gliosis were detected in the brain of a KA-treated monkey. Conclusions: Through a modified protocol of unilateral repetitive intra-amygdala KA injections, a rhesus monkey model with similar behavioral and brain electrical features as TLE was developed.
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