Journal
CARBOHYDRATE POLYMERS
Volume 175, Issue -, Pages 628-635Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.08.029
Keywords
Chitosan-genipin films; Decolorization; Laccase; Mediator; Hydrogen peroxide; Active materials
Categories
Funding
- FCT/MEC [UID/CTM/50011/2013]
- FEDER [PT2020]
- FCT [SFRH/BPD/104712/2014, SFRH/BPD/100627/2014, IF/00300/2015]
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Chitosan-genipin films present a bluish-green color due to the conjugated double bonds formed when monomeric or dimeric genipin residues bridge chitosan. This phenomenon limits their use when colorless materials are required. In this work, a two-step oxidation strategy was developed aiming to remove color from chitosan-genipin films while preserving their functional properties. A combined system using the recombinant CotA laccase from Bacillus subtilis mediated by 2,2'-azinobis-(3-ethylbenzothiazoline6-sulphonic acid) (ABTS) followed by a H2O2 oxidation step was settled. ABTS boosted the laccase performance resulting in light brown chitosan-genipin films that were further decolorized upon immersion in a 5% H2O2 solution at pH 11.0 and 40 degrees C for 30 min. The applied methodology leads to films that sustain both acidic stability and antioxidant capacity of the pristine films. Overall, the combined CotA laccase-ABTS/hydrogen peroxide developed methodology efficiently produce active and decolorized chitosan-genipin films with potential for application as eco-friendly transparent material which may have application as food packaging. (C) 2017 Elsevier Ltd. All rights reserved.
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