Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 159, Issue -, Pages 159-165Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2017.07.050
Keywords
Thermoresponsive polymer; Cell detachment; Poly(N-isopropylacrylamide); Crosslinking and tissue engineering
Funding
- Irish Research Council under the Embark scheme [RCS810]
- National University of Ireland Galway
- Irish Government's Program for Research in Third Level Institutions, Cycles 4
- Russian Science Foundation [15-15-00132]
- Irish Government's Program for Research in Third Level Institutions, Cycles 5
- Russian Science Foundation [15-15-00132] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
The primary aim of this investigation was to determine the biocompatibility and cell culture potential of a newly designed class of thermoresponsive polymers. The attractiveness of these polymers lies in the fact that they swell rather than dissolve when the temperature is reduced below their respective lower critical solution temperature, due to the incorporation of octadecyl methacrylate (ODMA). The ODMA monomer acts as a physical crosslinker, preventing polymer dissolution upon temperature reduction. Two polymers were studied in this investigation poly(N isorpoylacrylamide (NIPAm)(99.25%)-co-ODMA(0.75%)) and poly(NIPAm(65%)-co-N-tert-butylacrylamide (NtBAm)(34.25%)-co-ODMA(0.75%)). Thin thermoresponsive films of the polymers were prepared via spin coating. 3T3 cells were then seeded on the prepared films and cell viability was assessed quantitatively through cell viability and activity assays and qualitatively by light microscopy. Cells were successfully seeded and grown on the poly(NIPAm-co-ODMA) and poly(NIPAm-co-NtBAm-co-ODMA) copolymer films after film modification with cell adhesion promoters (CAPs). Cell sheets successfully detached from the CAP coated poly(NIPAm-co-ODMA) platforms upon temperature reduction. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available