Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 101, Issue 22, Pages 8077-8088Publisher
SPRINGER
DOI: 10.1007/s00253-017-8493-0
Keywords
Taxonomy; DNAextraction; Liquid-liquid extraction; Solid phase extraction; Microfluidics
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Funding
- NanoMalaysia Institute for Innovative Technology (NanoMite) [9012 00006]
- Long Term Research Grant (LRGS), Ministry of Education, Malaysia
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This review covers a developmental progression on early to modern taxonomy at cellular level following the advent of electron microscopy and the advancement in deoxyribonucleic acid (DNA) extraction for expatiation of biological classification at DNA level. Here, we discuss the fundamental values of conventional chemical methods of DNA extraction using liquid/liquid extraction (LLE) followed by development of solid-phase extraction (SPE) methods, as well as recent advances in microfluidics device-based system for DNA extraction on-chip. We also discuss the importance of DNA extraction as well as the advantages over conventional chemical methods, and how Lab-on-a-Chip (LOC) system plays a crucial role for the future achievements.
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