Journal
CARBOHYDRATE POLYMERS
Volume 175, Issue -, Pages 63-74Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.07.063
Keywords
Glycosaminoglycans; Chondroitin sulfate; Alginate Cartilage tissue engineering; Injectable; Zein
Categories
Funding
- Nano Mission of the Department of Science AMP
- Technology (DST), Government of India [SR/NM/PG-16/2007]
- FIST program of the Department of Science AMP
- Technology (DST), Government of India [SR/FST/ST/LSI-453/2010]
- Drugs AMP
- Pharmaceuticals Research Programme, DST, India
- SASTRA University
- Innovation in Science Pursuit for Inspired Research (INSPIRE), DST, India [IF120692]
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Articular hyaline cartilage regeneration remains challenging due to its less intrinsic reparability. The study develops injectable biphasic semi-interpenetrating polymer networks (SIPN) hydrogel impregnated with chondroitin sulfate (ChS) nanoparticles for functional cartilage restoration. ChS loaded zein nanoparticles (-150 nm) prepared by polyelectrolyte-protein complexation were interspersed into injectable SIPNs developed by blending alginate with poly(vinyl alcohol) and calcium crosslinking. The hydrogel exhibited interconnected porous microstructure (39.9 +/- 5.8 pan pore diameter, 57.7 +/- 5.9% porosity), 92% swellability and >350 Pa elastic modulus. Primary chondrocytes compatibility, chondrocyte-matrix interaction with cell-cell clustering and spheroidal morphology was demonstrated in ChS loaded hydrogel and long-term (42 days) proliferation was also determined. Higher fold expression of cartilage -specific genessox9, aggrecan and collagen-II was observed in ChS loaded hydrogel while exhibiting poor expression of collagen-I. Immunoblotting of aggregan and collagen II demonstrate favorable positive influence of ChS on chondrocytes. Thus, the injectable biphasic SIPNs could be promising composition-mimetic substitute for cartilage restoration at irregular defects. (C) 2017 Elsevier Ltd. All rights reserved.
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