Journal
BIORESOURCE TECHNOLOGY
Volume 244, Issue -, Pages 151-159Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.07.108
Keywords
Rice milling by-products; Alkaline peroxide pre-treatment; Enzymatic saccharification; Consolidated bioprocessing; Multiple waste streams co-fermentation
Funding
- University of Padova [GRIC120EG8, 60A08-0924/15, DOR1657411/16, DOR1715524/17, DOR1728499/17]
- Regione Veneto (PSR) [Misura 124, 2307660]
- [ZA11MO2]
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This work describes the feasibility of using rice milling by-products as feedstock for bioethanol. Starch-rich residues (rice bran, broken, unripe and discolored rice) were individually fermented (20% w/v) through Consolidated Bioprocessing by two industrial engineered yeast secreting fungal amylases. Rice husk (20% w/v), mainly composed by lignocellulose, was pre-treated at 55 degrees C with alkaline peroxide, saccharified through optimized dosages of commercial enzymes (Cellic (R) CTec2) and fermented by the recombinant strains. Finally, a blend of all the rice by-products, formulated as a mixture (20% w/v) according to their proportions at milling plants, were co-processed to ethanol by optimized pre-treatment, saccharification and fermentation by amylolytic strains. Fermenting efficiency for each by-product was high (above 88% of the theoretical) and further confirmed on the blend of residues (nearly 52 g/L ethanol). These results demonstrated for the first time that the co-conversion of multiple waste streams is a promising option for second generation ethanol production.
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