Journal
ANALYTICAL METHODS
Volume 9, Issue 31, Pages 4592-4598Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ay01523b
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Funding
- FONDECYT [1160942]
- CONICYT [63140157]
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The analytical test-system for cocaine, benzoylecgonine and norcocaine was developed in the ELISA format using molecularly imprinted polymeric nanoparticles (nanoMIPs) as synthetic recognition elements that were produced using a solid-phase synthesis approach. The experimental conditions of the assay were optimized using a Box-Behnken experimental design protocol. The detection of free cocaine and its metabolites was performed using a competitive binding assay in the model samples and in blood plasma. There was no cross-reactivity of the developed assay towards paracetamol and caffeine. The developed assay had a picomolar limit of detection of cocaine (LOD=4.24 pM), which was almost three orders of magnitude lower than the LOD expected from the commercial antibodybased ELISA (3.3 nM), and other attractive features of a new assay included a long shelf-life, lower economic cost and a short production time. Therefore, it is possible to state that nanoMIPs have the potential to become the recognition elements of choice for the development of a new generation of test-systems and sensors.
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