Journal
VIRUS RESEARCH
Volume 242, Issue -, Pages 49-57Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2017.08.012
Keywords
Metagenomics; Reoviridae; Viral discovery; Mosquito; Deep sequencing; Orbivirus
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Funding
- Blood Systems Research Institute
- Grifols Diagnostic Solutions, Inc.
- NIH [HHSN272701000040I/HHSN2720004/D04, R24 AI 120942]
- Institute for Human Infections and Immunity
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Eleven viral isolates derived mostly in albopictus C6/36 cells from mosquito pools collected in Southeast Asia and the Americas between 1966 and 2014 contained particles with electron microscopy morphology typical of reoviruses. Metagenomics analysis yielded the near complete genomes of three novel reoviruses, Big Cypress orbivirus, Ninarumi virus, and High Island virus and a new tetravirus, Sarawak virus. Strains of previously characterized Sathuvarachi, Yunnan, Banna and Parry's Lagoon viruses (Reoviridae), Bontang virus (Mesoniviridae), and Culex theileri flavivirus (Flaviviridae) were also characterized. The availability of these mosquito virus genomes will facilitate their detection by metagenomics or PCR to better determine their geographic range, extent of host tropism, and possible association with arthropod or vertebrate disease.
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