Journal
VIRUS GENES
Volume 53, Issue 4, Pages 667-671Publisher
SPRINGER
DOI: 10.1007/s11262-017-1470-y
Keywords
High-throughput sequencing; Virus discovery; Luteoviridae; Grapevine enamovirus-1; GEV-1
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Funding
- Embrapa (Empresa Brasileira de Pesquisa Agropecuaria, Brazil) [02.13.14.002]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil)
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In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remarkable identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3' untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus.
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