Journal
VIROLOGY
Volume 500, Issue -, Pages 169-177Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.10.025
Keywords
West Nile virus; Capsid; DDX56; RNA helicase; Virus assembly; Endoplasmic reticulum; Flavivirus; Super-resolution microscopy
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Funding
- Canadian Institutes of Health Research (CIHR) [CIHR MOP-125903]
- province of Alberta
- Canada Foundation for Innovation
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Flaviviruses, including the human pathogen, West Nile virus (WNV), are known to co-opt many host factors for their replication and propagation. To this end, we previously reported that the nucleolar DEAD-box RNA helicase, DDX56, is important for production of infectious WNV virions. In this study, we show that WNV infection results in relocalization of DDX56 from nucleoli to virus assembly sites on the endoplasmic reticululm (ER), an observation that is consistent with a role for DDX56 in WNV virion assembly. Super-resolution microscopy revealed that capsid and DDX56 localized to the same subcompartment of the ER, however, unexpectedly, stable interaction between these two proteins was only detected in the nucleus. Together, these data suggest that DDX56 relocalizes to the site of virus assembly during WNV infection and that its interaction with WNV capsid in the cytoplasm may occur transiently during virion morphogenesis.
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