Journal
VIROLOGY
Volume 511, Issue -, Pages 95-105Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.08.026
Keywords
Coronavirus; MERS-CoV; Nspl; RNA recognition; Viral replication
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Funding
- Takeda Science Foundation
- Japan Agency for Medical Research and Development, AMED
- [16K08811]
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MERS-CoV is the only lethal human CoV still endemic in the Arabian Peninsula and neither vaccine nor therapeutics against MERS-CoV infection is available. The nspl of CoV is thought to be a major virulence factor because it suppresses protein synthesis through the degradation of host mRNA. In contrast, viral RNA circumvents the nspl-mediated translational shutoff for an efficient propagation. In this study, we identified amino acid residue in MERS-CoV nspl that differ from those of SARS-CoV nspl, and that appear to be crucial for circumventing the translational shutoff. In addition, reverse genetics analysis suggested the presence of a cisacting element at the 5 '-terminus of the nspl-coding region, which contributes to the specific recognition of viral RNA that is required for an efficient viral replication. Our results suggest the CoVs share a common mechanism for circumventing the nsp 1-mediated translational shutoff.
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