Journal
VIROLOGY
Volume 509, Issue -, Pages 185-194Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2017.06.013
Keywords
Porcine epidemic diarrhea virus (PEDV); Spike; Neutralizing antibodies
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Funding
- South Dakota Animal Disease Research & Diagnostic Laboratory
- South Dakota Agricultural Experiment Station (AES Hatch Project) [SDH00392, SDOOH517-14]
- Boehringer Ingelheim Vetmedica Inc [3P5742]
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The porcine epidemic diarrhea virus (PEDV) spike (S) protein is the major target of neutralizing antibodies against PEDV. Here immunodominant neutralizing epitopes of PEDV were identified using a panel of S-specific monoclonal antibodies (mAbs). Ten of eleven S-specific mAbs successfully neutralized PEDV infectivity in vitro. Notably, epitope mapping by peptide ELISAs revealed that nine of these mAbs recognized linear neutralizing epitopes located in the N-terminus of the S2 glycoprotein subunit (amino acids [aa] 744-759, 747-774 and/or 756-771). Additionally, one mAb recognized a neutralizing epitope located in the C-terminus of S2 (aa 13711377), while only one neutralizing mAb reacted against a region of the Si glycoprotein subunit (aa 499-600). Notably, mAbs that recognized epitopes within the S2 subunit presented the highest neutralizing activity against PEDV. Together these results indicate that the S2 glycoprotein subunit contains major antigenic determinants and, perhaps, the immunodominant neutralizing epitopes of PEDV.
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