Journal
VIROLOGY
Volume 500, Issue -, Pages 82-90Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.09.030
Keywords
KHV; ORF6 protein; B cell; Koi; Latent herpesvirus; Sumoylation
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Funding
- Associated Koi Clubs of America
- Office of the Director of the National Institutes of Health T32 training grant [RR023917, T32OD011020]
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Koi herpesvirus (KHV) is highly pathogenic to Cyprinus carpio. KHV can also become latent in recovered fish and reactivate from latency under stressful conditions. Understanding KHV latency is important for development of strategies against herpesvirus latent infection. Our previous studies found KHV ORF6 mRNA is detectable during latent infection. In this study, ORF6 protein expression was investigated by a polyclonal antibody specific to ORF6 peptide. Positive staining by an immunofluorescence assay was observed in both KHV infected CCB (common carp brain) cells and white blood cells (WBCs) from recovered KHV+ koi. Proteins at the expected size, 68 kDa, and several different sizes can be detected during productive infection. Five potential sumoylation sites were identified in the ORF6 protein. Our study demonstrated that ORF6 protein is expressed in both productive infection and latent infection and may have different post-translational modifications during productive infection.
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