Journal
BIO-PROTOCOL
Volume 7, Issue 21, Pages -Publisher
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2591
Keywords
Skeletal muscle; Myoblast isolation; Tissue dissociation; Fluorescence-activated cell sorting (FACS)
Categories
Funding
- Muscular Dystrophy Association [479606]
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [1R01AR069582-01]
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Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.
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