Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 23, Issue 63, Pages 16026-16033Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201703232
Keywords
coordination polymers; heme proteins; mesoporous materials; solid-phase extraction; zinc
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Funding
- National Natural Science Foundation of China [21303010, 21273029]
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A composite material, ZnO@MC, has been synthesized successfully by calcination using a one-dimensional zinc-based coordination polymer as the precursor. In ZnO@MC, ZnO particles with a size of about 5-8 nm are dispersed evenly in a mesoporous carbon matrix. Adsorption experiments at pH 6.8 with 2 mg ZnO@MC as adsorbent illustrated an adsorption efficiency of 92.3% in 5 mL hemoglobin (Hb) solution with a concentration of 100 mg L-1. In contrast, the adsorption of bovine serum albumin can almost be ignored under the same conditions. The selectivity originates from a strong Zn-II-histidine interaction between ZnO@MC and hemoglobin. The adsorption behavior of hemoglobin on ZnO@MC fits the Temkin model perfectly with a capacity as high as 11646 mg g(-1). The hemoglobin adsorbed on the composite material can be eluted easily with sodium dodecyl sulfate stripping reagent with an extraction efficiency of 87.7%. Circular dichroism spectra and protein activity studies suggest the structure and biological activity of hemoglobin is the same before and after the adsorption/desorption experiment. Finally, the ZnO@MC composite material was employed to extract hemoglobin from human whole blood without any pretreatment, and gave a very satisfactory result.
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