4.8 Article

Self-cascade reaction catalyzed by CuO nanoparticle-based dual-functional enzyme mimics

Journal

BIOSENSORS & BIOELECTRONICS
Volume 97, Issue -, Pages 21-25

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.05.037

Keywords

Cysteine oxidase; Peroxidase; Enzyme mimics; Artificial enzymatic cascade reaction; Cupric oxide nanoparticles; Cysteine

Funding

  1. National Natural Science Foundation of China [21175023, 21675024, 81371902]
  2. Program for New Century Excellent Talents in University [NCET-12-0618]
  3. Program for Innovative Leading Talents in Fujian Province [2016B016]
  4. Joint Funds for the Innovation of Science and Technology, Fujian Province [2016Y9056]

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It is desirable but challenging to assemble various biomimetic properties into a functional catalytic cascade system. In this work, cupric oxide nanoparticles were investigated as oxidase mimics for the aerobic oxidation of cysteine to cystine with the generation of hydrogen peroxide. Coupling this property with the peroxidase-like activity of CuO nanoparticles, we constructed a self-organized cascade reaction system based on a single-component nanozyme, which includes the oxidation of cysteine to yield cystine and hydrogen peroxide and the hydrogen peroxide-mediated oxidation of terephthalic acid to produce a fluorescence change. Based on this artificial enzymatic cascade reaction system, a fluorometric assay method with a low detection limit of 6.6 nM was established for cysteine determination. This platform was then applied for the detection of cysteine in pharmaceutical products and human plasma, which yielded satisfactory results. Our investigations open up a new route and holds promise for the development and applications of multifunctional nanomaterials as enzyme mimics.

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