Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 127, Issue -, Pages 43-52Publisher
ELSEVIER
DOI: 10.1016/j.bej.2017.07.011
Keywords
Immobilized enzyme; Magnetic microspheres; Mesoporous materials; Penicillin G acylase; Hyperbranched amine
Funding
- State of China [51433008]
- International Cooperation and Exchanges NSFC [51711530233]
- Fundamental Research Funds for the Central Universities
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Fe3O4@SiO2@m-SiO2 magnetic mesoporous (SmSMM) microspheres were synthesized by two steps Stbber hydrolysis. The surface of the SmSMM microspheres were modified with gamma-(2,3-epoxypropoxy)propytrimethoxysilane (KH560) and dendritic hyperbranched amine (DHA) to obtain SmSMM-DHA microspheres, which were used as carriers for the immobilization of penicillin G acylase (PGA). The diameter of SmSMM-DHA microspheres was around 500 nm and the specific saturation magnetization was 61.25 emu g(-1). The advantages of the as-prepared carries were large specific surface area and high amino content, the values were 323.1 m(2) g(-1) and 2.1 mmol g(-1), respectively. The influences of the factors on amino content, immobilization rate and enzyme activity were investigated. The research results of PGA immobilized on SmSMM-DHA microspheres indicated that compared with free enzyme, the immobilized PGA showed excellent pH and thermo stability. Meanwhile, after being used for ten times, the enzyme activity retention rate was 94.5%. The designed immobilized enzyme shows potential application value in the synthesis of antibiotics. (C) 2017 Published by Elsevier B.V.
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