Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 89, Pages 12116-12119Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc03462h
Keywords
-
Categories
Funding
- EPSRC [EP/M023664/1, EP/H003789/1]
- Royal Society
- Higher Committee for Education Development in Iraq
- Access to Research Infrastructures activity in the 7th Framework Programme of the EC [261863]
- Engineering and Physical Sciences Research Council [EP/M023664/1, EP/H003789/1] Funding Source: researchfish
- EPSRC [EP/M023664/1, EP/H003789/1] Funding Source: UKRI
Ask authors/readers for more resources
The quadrupolar interaction experienced by the spin-1 N-14 nucleus is known to be extremely sensitive to local structure and dynamics. Furthermore, the N-14 isotope is 99.6% naturally abundant, making it an attractive target for characterisation of nitrogen-rich biological molecules by solid-state NMR. In this study, dynamic nuclear polarization (DNP) is used in conjunction with indirect N-14 detected solid-state NMR experiments to simultaneously characterise the quadrupolar interaction at multiple N-14 sites in the backbone of the microcrystalline protein, GB3. Considerable variation in the quadrupolar interaction (>700 kHz) is observed throughout the protein backbone. The distribution in quadrupolar interactions observed reports on the variation in local backbone conformation and subtle differences in hydrogen-bonding; demonstrating a new route to the structural and dynamic analysis of biomolecules.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available