Journal
MICROBIAL CELL
Volume 4, Issue 10, Pages 342-361Publisher
SHARED SCIENCE PUBLISHERS OG
DOI: 10.15698/mic2017.10.594
Keywords
Candida albicans; stress; mitochondria; morphogenesis; heat shock; mitochondrial processing peptidases
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Funding
- Sir Henry Wellcome Postdoctoral Fellowship (Wellcome Trust) [096072]
- Canada Research Chair in Microbial Genomics and Infectious Disease
- Canadian Institutes of Health Research (CIHR) [MOP-119520, MOP-86452]
- National Institutes of Health grant [5R01GM108975]
- CIHR Foundation Grant [FDN143301]
- Genome Canada Genomics Innovation Network (GIN) Node and Technical Development Grants
- Canada Research Chair in Functional Proteomics
- TD Bank Health Research Fellowship at the Lunenfeld-Tanenbaum Research Institute
- Cancer Research Society
- CIHR Frederick Banting and Charles Best Canada Graduate Scholarship
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Mitochondria underpin metabolism, bioenergetics, signalling, development and cell death in eukaryotes. Most of the similar to 1,000 yeast mitochondrial proteins are encoded in the nucleus and synthesised as precursors in the cytosol, with mitochondrial import facilitated by molecular chaperones. Here, we focus on the Hsp40 chaperone Ydj1 in the fungal pathogen Candida albicans, finding that it is localised to both the cytosol and outer mitochondrial membrane, and is required for cellular stress responses and for filamentation, a key virulence trait. Mapping the Ydj1 protein interaction network highlighted connections with co-chaperones and regulators of filamentation. Furthermore, the mitochondrial processing peptidases Mas1 and Mas2 were highly enriched for interaction with Ydj1. Additional analysis demonstrated that loss of MAS1, MAS2 or YDJ1 perturbs mitochondrial morphology and function. Deletion of YDJ1 impairs import of Su9, a protein that is cleaved to a mature form by Mas1 and Mas2. Thus, we highlight a novel role for Ydj1 in cellular morphogenesis, stress responses, and mitochondrial import in the fungal kingdom.
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