Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 183, Issue 3, Pages 765-777Publisher
SPRINGER
DOI: 10.1007/s12010-017-2462-z
Keywords
Waste biotechnology; Biopolymer dressing; Biocompatibility; Zinc oxide; Antimicrobial
Funding
- CAPES
- CNPq
- Financiadora de Estudos e Projetos-FINEP, of the Brazilian government [01.13.0220.00]
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco FACEPE
Ask authors/readers for more resources
In this work, chitosan-based films containing gelatin and chondroitin-4-sulfate (C4S) with and without ZnO particles were produced and tested in vitro to investigate their potential wound healing properties. Chitosans were produced from shrimp-head processing waste by alkaline deacetylation of chitin to obtain chitosans differing in molecular weight and degree of deacetylation (80 +/- 0.5%). The film-forming solutions (chitosan, C4S and gelatin) and ZnO suspension showed no toxicity towards fibroblasts or keratinocytes. Chitosan was able to agglutinate red blood cells, and film-forming solutions induced no hemolysis. Film components were released into solution when incubated in PBS as demonstrated by protein and sugar determination. These data suggest that a stable, chitosan-based film with low toxicity and an ability to release components would be able to establish a biocompatible microenvironment for cell growth. Chitosan-based films significantly increased the percentage of wound healing (wound contraction from 65 to 86%) in skin with full-thickness excision when compared with control (51%), after 6 days. Moreover, histological analysis showed increased granulation tissue in chitosan and chitosan/gelatin/C4S/ZnO films. Chitosan-based biopolymer composites could be used for improved biomedical applications such as wound dressings, giving them enhanced properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available