Journal
CARBOHYDRATE POLYMERS
Volume 178, Issue -, Pages 209-220Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.09.050
Keywords
Pectin; Polysaccharide; C57BL/KsJdb/db mice; Type 2 diabetes mellitus; Hypoglycemic effect; Hepatic glycogen
Categories
Funding
- key New Drug Creation and Manufacturing Program in China [2012ZX09301001-003]
- National Natural Science Foundation of China [81373951]
Ask authors/readers for more resources
In this study, a particular pectic polysaccharide (FPLP) was extracted and purified from the fruits of Ficus pumila Linn. through boiling water extraction, alcohol precipitation, diethylaminoethyl-Sepharose Fast Flow chromatography and Superdex T G-75 gel filtration chromatography. Analysis of high-performance gel permeation chromatography, FTIR, GC-MS, methylation and 1D/2D NMR spectroscopy revealed that FPLP (Mw: 34.69 kDa) is a linear (1,4)-alpha-D-galacturonic acid binding 1.30% branched chain hexenuronic acid with 23.34% methyl esterification. Treatment with FPLP ameliorated hyperglycaemia in association with an improvement in hepatic glycogen metabolism in C57BL/KsJ db/db mice. The activation of IRS-1/PI3K/Akt/GSK3 beta/GS insulin signalling pathway and AMPK/GSK3 beta/GS signalling pathway and the regulation of glucokinase, phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expressions involved in hepatic glycogenesis and glycogenolysis were considered the therapeutic mechanisms of FPLP. These results provide a new insight for investigating the effects of pectic polysaccharides on blood glucose control and suggest that FPLP is a promising nutraceutical for treatment of T2DM.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available