Journal
BIORESOURCE TECHNOLOGY
Volume 245, Issue -, Pages 1407-1412Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.100
Keywords
Methanol; Non-methylotrophy; S. cerevisiae; Synthetic biology; Pyruvate
Funding
- China Postdoctoral Science Foundation [2016M601787]
- Postdoctoral Foundation of Jiangsu Province [1601043B]
- Key Science and Technology Project of Jiangsu Province [BE2016389]
- Jiangsu Province Natural Science Foundation for Youths [BK20140940]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Program for Changjiang Scholars and Innovative Research Team in University [06A-047]
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The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified nonmethylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes. (C) 2017 Elsevier Ltd. All rights reserved.
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