Journal
ARCHIVES OF VIROLOGY
Volume 162, Issue 12, Pages 3913-3917Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-017-3552-5
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Funding
- National Institutes of Health [R01AI114720]
- Wellcome Trust [106207]
- European Research Council (ERC) European Union [646891]
- European Research Council (ERC) [646891] Funding Source: European Research Council (ERC)
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We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounan, virus (53% identity, 70% similarity), respectively. Phylogenetic inference is in agreement with these findings. Potential programmed -1 ribosomal frameshifting sites were bioinformatically identified in the genomes of both viruses.
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