Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 80, Pages 11040-11043Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc06140d
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Funding
- National Natural Science Foundation of China [21335005, 21472120, 21622507]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT_15R43]
- Natural Science Basic Research Plan in Shaanxi Province [2015KJXX-22]
- Fundamental Research Funds for the Central Universities [GK201501003, GK201603039]
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A novel one-step microRNA assay is developed based on a target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism, which enables the accurate detection of as low as 100 aM (1 zmol) microRNA with simple one-step operation by using only one-type of DNA polymerase.
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