Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 40, Pages 34736-34743Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b12733
Keywords
graphitic carbon nitride; graphene; electrospun fibers; visible-light photocatalytic; neural stimulator
Funding
- Danish Council for Independent Research [DFF-7017-00185]
- EU H2020 RISE [2016-MNR4SCe11 734174]
- Aarhus University Research Foundation
- Carlsberg Foundation
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Light stimulation allows remote and spatiotemporally accurate operation that has been applied as effective, noninvasive means of therapeutic interventions. Here, visible-light neural stimulation of graphitic carbon nitride (g-C3N4), an emerging photocatalyst with visible-light optoelectronic conversion, was for the first time investigated. Specifically, g-C3N4 was combined with graphene oxide (GO) in a three-dimensional manner on the surfaces of electrospun polycaprolactone/gelatin (PG) fibers and functioned as a biocompatible interface for visible-light stimulating neuronal differentiation. The enhanced photocatalytic function of g-C3N4 was realized by spreading g-C3N4 on GO coated electrospun (PG) microfibers to improve both charge separation and surface area. Ascorbic acid (AA) was used in the cell culture medium not only as a photoexcited hole scavenger but also as a mediator of GO reduction to further improve the electrical conductivity. The successful coatings of g-C3N4, GO, and AA-mediated GO reduction were confirmed using scanning electron microscopy, photoluminescence, Raman spectroscopy, and X-ray photoelectron spectroscopy. Biocompatibility of g-C3N4 (0.01-0.9 mg/mL) to PC12 cells was confirmed by the lactate dehydrogenase (LDH) assay, Live Dead staining, and colorimetric cell viability assay CCK-8. Under a bidaily, monochromatic light stimulation at a wavelength of 450 nm at 10 mW/cm(2), a 18.5-fold increase of neurite outgrowth of PC12 was found on g-C3N4-coated fibers, while AA-reduced GO g-C3N4 hybrid brought a further 2.6-fold increase, suggesting its great potential as a visible-light neural stimulator that could optically enhance neural growth in a spatiotemporal-specific manner.
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